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colorimetric elisa kit  (Novus Biologicals)


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    Novus Biologicals colorimetric elisa kit
    a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by <t>ELISA.</t> Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.
    Colorimetric Elisa Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+creatine+kinase+mb+elisa+kit/pmc12948977-543-11-15?v=Novus+Biologicals
    Average 94 stars, based on 4 article reviews
    colorimetric elisa kit - by Bioz Stars, 2026-08
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    Images

    1) Product Images from "Activation of IRF3 in cardiomyocytes impairs mitochondrial oxidative function through PGC-1α inhibition and drives heart failure"

    Article Title: Activation of IRF3 in cardiomyocytes impairs mitochondrial oxidative function through PGC-1α inhibition and drives heart failure

    Journal: Nature Communications

    doi: 10.1038/s41467-026-69792-4

    a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by ELISA. Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.
    Figure Legend Snippet: a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by ELISA. Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.

    Techniques Used: Transgenic Assay, Expressing, Western Blot, Two Tailed Test, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Gene Expression



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    a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by <t>ELISA.</t> Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.
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    a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by <t>ELISA.</t> Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.
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    a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by <t>ELISA.</t> Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.
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    a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by ELISA. Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.

    Journal: Nature Communications

    Article Title: Activation of IRF3 in cardiomyocytes impairs mitochondrial oxidative function through PGC-1α inhibition and drives heart failure

    doi: 10.1038/s41467-026-69792-4

    Figure Lengend Snippet: a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by ELISA. Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.

    Article Snippet: Quantitative determination of CK-MB levels was performed in plasma samples using colorimetric ELISA kit (#NBP2-75312, Novus Biologicals) following manufacturer’s instructions.

    Techniques: Transgenic Assay, Expressing, Western Blot, Two Tailed Test, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Gene Expression