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colorimetric elisa kit  (Novus Biologicals)


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    Novus Biologicals colorimetric elisa kit
    a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by <t>ELISA.</t> Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.
    Colorimetric Elisa Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+creatine+kinase+mb+elisa+kit/Mouse+Creatine+Kinase+MB+ELISA+Kit+(Colorimetric)/pmc12948977-543-11-15
    Average 94 stars, based on 4 article reviews
    colorimetric elisa kit - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Activation of IRF3 in cardiomyocytes impairs mitochondrial oxidative function through PGC-1α inhibition and drives heart failure"

    Article Title: Activation of IRF3 in cardiomyocytes impairs mitochondrial oxidative function through PGC-1α inhibition and drives heart failure

    Journal: Nature Communications

    doi: 10.1038/s41467-026-69792-4

    a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by ELISA. Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.
    Figure Legend Snippet: a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by ELISA. Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.

    Techniques Used: Transgenic Assay, Expressing, Western Blot, Two Tailed Test, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Gene Expression

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Pgam5-mediated PHB2 dephosphorylation contributes to endotoxemia-induced myocardial dysfunction by inhibiting mitophagy and the mitochondrial unfolded protein response.
    Article Snippet: .. 19 https://www.ijbs.com 4660 Mouse TNT/Troponin T ELISA Kit (F55154, LifeSpan BioSciences), Mouse Brain Natriuretic Peptide (BNP) ELISA Kit (CSB-E07971m, CUSABIO), and Mouse Creatine Kinase MB ELISA Kit (NBP2-74312, Novus Biologicals) were used to determine the activity/ concentration of Bax, Bcl-2, Gpx4, Ptx3, Txnrd2, BNP, TnT, and CK-MB [47]. .. HL-1 cells were cultured in DMEM (Gibco, Agawam, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) at 37°C in a 5% CO2 atmosphere.

    Article Title: Influenza virus replication in cardiomyocytes drives heart dysfunction and fibrosis
    Article Snippet: Quantification of serum IL-6, IL-8, IL-1β, and TNFα was performed using a mouse V-PLEX Proinflammatory Panel 1 kit (Meso Scale Diagnostics) and was analyzed by the OSU Center for Clinical and Translational Science. .. Creatine kinase quantification was performed using the Mouse Creatine Kinase MB ELISA Kit (Novus Biologicals). ..

    Activity Assay:

    Article Title: Pgam5-mediated PHB2 dephosphorylation contributes to endotoxemia-induced myocardial dysfunction by inhibiting mitophagy and the mitochondrial unfolded protein response.
    Article Snippet: .. 19 https://www.ijbs.com 4660 Mouse TNT/Troponin T ELISA Kit (F55154, LifeSpan BioSciences), Mouse Brain Natriuretic Peptide (BNP) ELISA Kit (CSB-E07971m, CUSABIO), and Mouse Creatine Kinase MB ELISA Kit (NBP2-74312, Novus Biologicals) were used to determine the activity/ concentration of Bax, Bcl-2, Gpx4, Ptx3, Txnrd2, BNP, TnT, and CK-MB [47]. .. HL-1 cells were cultured in DMEM (Gibco, Agawam, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) at 37°C in a 5% CO2 atmosphere.

    Concentration Assay:

    Article Title: Pgam5-mediated PHB2 dephosphorylation contributes to endotoxemia-induced myocardial dysfunction by inhibiting mitophagy and the mitochondrial unfolded protein response.
    Article Snippet: .. 19 https://www.ijbs.com 4660 Mouse TNT/Troponin T ELISA Kit (F55154, LifeSpan BioSciences), Mouse Brain Natriuretic Peptide (BNP) ELISA Kit (CSB-E07971m, CUSABIO), and Mouse Creatine Kinase MB ELISA Kit (NBP2-74312, Novus Biologicals) were used to determine the activity/ concentration of Bax, Bcl-2, Gpx4, Ptx3, Txnrd2, BNP, TnT, and CK-MB [47]. .. HL-1 cells were cultured in DMEM (Gibco, Agawam, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) at 37°C in a 5% CO2 atmosphere.



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    Cusabio creatine kinase myocardial band
    a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by <t>ELISA.</t> Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.
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    a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by <t>ELISA.</t> Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.
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    a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by <t>ELISA.</t> Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.
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    a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by ELISA. Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.

    Journal: Nature Communications

    Article Title: Activation of IRF3 in cardiomyocytes impairs mitochondrial oxidative function through PGC-1α inhibition and drives heart failure

    doi: 10.1038/s41467-026-69792-4

    Figure Lengend Snippet: a Representative model showing strategy of CMI3OE transgenic mouse generation. Irf3-2D fl/fl mice were bred with αMHCMerCreMer (αMHCMCM) mice to generate tamoxifen inducible cardiomyocyte-specific CMI3OE ( Irf3-2D fl/αMHCMCM) mice. b Schematic representation of cardiomyocyte-specific expression of murine IRF3-2D by oral gavage of low dose tamoxifen (30 mg/kg) to induce MerCreMer-mediated excision of cardiomyocyte-specific floxed cassette in 12wk old male mice. c Gross morphology of αMHCMCM (Cre) and CMI3OE male mice at 12wks of age. d Kaplan-Meier curve showing survival of CMI3OE compared to αMHCMCM (Cre) mice. e Western blot showing protein levels of IRF3 in the cardiac ventricular tissue, n = 3 per group, biological replicates. f Quantification of the Western blot shown in Fig. 3e, n = 3 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test ***P = 2 × 10 -5 . g mRNA expression of Irf3 and its target genes in the cardiac ventricular tissue determined by qPCR, n = 7 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, Irf3 : *** P = 1.8 × 10 -8 ; Ifnβ : * P < 0.0280; Ifit1 : *** P < 1.5 × 10 -9 ; Ifit2 : *** P < 0.0005; Ifit3 : *** P < 0.0002; Rsad2 : *** P < 9.9 × 10 -7 ; Ccl2 : ** P < 0.0030. h-j Plasma cytokine levels in CMI3OE mice determined by ELISA. Samples used are biological replicates. Data analyzed by unpaired two-tailed Student’s t test, CCL2: * P = 0.0430 [ n = 5 per group]; TNFα: * P = 0.0229 [ n = 5 per group]; IL-6: * P = 0.0532 [ n = 6 (Cre), n = 9 (CMI3OE)]. k Body weight measured in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. l Representative echocardiography images from αMHCMCM (Cre) and CMI3OE mice. m – q Cardiac function in αMHCMCM (Cre) and CMI3OE mice determined by echocardiography, n = 5 per group, biological replicates. Data analyzed by unpaired two-tailed Student’s t test, %EF: *** P = 2.3 × 10 -5 ; %FS: *** P = 5.8 × 10 -5 ; LVPWd: * P = 0.0448; LVmass/BW: * P = 0.0533. Cardiac function was measured in three independent cohorts with similar results. r Heart weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group, data analyzed by unpaired two-tailed Student’s t test, * P = 0.0399. s Lung weight to tibia length determined in αMHCMCM (Cre) and CMI3OE mice, n = 5 per group. Survival curve, gene expression analysis, and echocardiography experiments were performed using independent cohorts. Data in all panels are represented as mean ± SEM. Source data are provided as a Source data file.

    Article Snippet: Quantitative determination of CK-MB levels was performed in plasma samples using colorimetric ELISA kit (#NBP2-75312, Novus Biologicals) following manufacturer’s instructions.

    Techniques: Transgenic Assay, Expressing, Western Blot, Two Tailed Test, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Gene Expression